Website for surgical instrument cleaning detergents. The 4 enzymes needed for high performance surgical instrument cleaners are listed below with the specific surgical instrument cleaning function they perform. Lipase Enzymes: to breakdown fat from surgical instruments, to cleave fatty acid residue from the glycerol residue in a neutral fat or a phospholipid. Amylase Enzymes: to breakdown starch from surgical instruments, to catalyze the hydrolysis of starch to sugar to produce carbohydrate derivatives. Carbohydrase Enzymes: to breakdown starch to a lower level from surgical instruments, to catalyze the hydrolysis of higher carbohydrates to lower forms. Protease Enzymes: to breakdown blood from surgical instruments, including the proteinases and peptidases, to catalyze the hydrolytic breakdown of proteins. Surgical Instrument Cleaners must deliver these 4 enzymes or they cannot break down and remove all forms of proteinaceous bioburden from surgical instruments. What specific functions do surgical instrument cleaning enzymes perform?
Enzyme Detergent Surgical Instrument Detergent
Surgical Instrument Cleaners
Enzyme detergent surgical instrument cleaners are designed for thoroughly cleaning surgical instrument cleaner in less cleaning time and at a lower cost.
The use of enzymes of various compositions and concentrations, in surgical instrument detergents is becoming common. Over half of all surgical instrument detergents presently available contain some level of enzymes. The enzymatic enzyme surgical instrument detergent  industry is the largest single market for enzymes, at rate of 25 - 30% of total sales. Details of which enzymes are used within enzymatic enzyme surgical instrument detergents and the ways in which they are best used, are rarely been published.  Enzymatic enzyme surgical instrument detergents should: remove proteinaceous bioburden, dissolve mineral encrustation, remove stains, and enhance the “passive layer” of stainless steel. Enzymatic enzyme surgical instrument detergents should be “free rinsing” to render a residue free surface and should be designed to condition the source water to counter the potential pitting and staining effects of hard water. The primary goal of surgical instrument cleaning is to remove soil (proteinaceous bioburden) which includes proteins, starches and lipids.  This requires 4 enzymes ( Lipase Enzymes, Amylase Enzymes, Carbohydrase Enzymes and Protease Enzymes) for cleaning surgical instruments cleaner and faster with lower costs.
Surgical Instrument Cleaning Detergent Lubricants cut cleaning costs.
Enzymatic cleaner enzyme detergent surgical instrument cleaner product claims of "fast acting enzyme" and "strongest enzyme" are typically erroneous. Enzymes all act at the same speed and are of equal strength. The application and the dilution rates determine the speed and the strength as it relates to surgical instrument cleaning outcomes. Unlike detergents, higher concentrations of enzymes will deliver higher levels of surgical instrument cleaning. Applying the enzymes in a foam will deliver a thicker lay than applying enzymes with a spray. The thicker layer allows more of the enzymes to come in contact with the bioburden being cleaned from the surgical instruments.  
Enzyme Surgical Instrument Detergent
Common misunderstandings exist pertaining to the application of enzymatic enzyme detergent surgical instrument cleaners including the times and temperatures for optimal cleaning outcomes. There are parameters for the use of enzymatic enzyme detergents that can render a high probability of excellent cleaning outcomes.
Enzyme Surgical Instrument Cleaner 
It is commonly stated that enzymes “clean”. Enzymes alone do not “clean”. The primary function of enzymes is to break down soil, usually proteinaceous bioburden. For a cleaning product to “clean” surfactants are necessary that will remove the soil from the surface. A combination of enzymes and detergents is necessary for “cleaning”. Enzymes and detergent combination products are referred to as “enzymatic enzyme detergents” or "all-in-one enzymatic enzyme detergents" surgical instrument cleaners. The optimal temperature for maximum enzymatic enzyme detergent surgical instrument cleaner performance peaks at 137 degrees Fahrenheit (58.33 degree Celsius). The detergent components typically peak performance at 185 degrees Fahrenheit (85 degree Celsius). The peak performance for the enzymes and the detergents represent the top of a bell curve with increasing and decreasing enzymatic enzyme detergent performance to each side the peak.
Enzyme Detergent Surgical Instrument Detergent
surgical instrument cleaners 
Dosage rate claims as to the cleaning results promised from very small dosage rates of enzymatic enzyme detergent can be misleading. Due to the wide variance of potential applications, the only dosage rates that can be attributed to a level of enzymatic enzyme detergent cleaning performance are those dosage rates that have been observed within your facility to render the cleaning outcomes required. The optimal enzymatic enzyme detergent dosage rate (amount diluted per quantity of carrier solution, usually ounces per gallon of neutral pH water) is a function of the types of enzymes and the concentration level of enzymes within the enzymatic enzyme detergent product. Detergents can render inferior performance with higher levels of concentration. Enzymes can render better performance at higher levels of concentration. To determine the optimal (lowest dosage with highest cleaning result) amount of enzymatic enzyme detergent to use, begin with the dosage recommended by the manufacture and then titrate the dosage higher or lower based on your cleaning outcomes. The cost of enzymatic enzyme detergents products is reconciled by their performance and your cost benefit analysis will be reconciled by the savings in the quantity of enzymatic enzyme detergents used.
Enzyme Surgical Instrument Cleaner Detergent
surgical instrument cleaners
The optimal application time for maximum enzymatic enzyme detergent cleaning performance depends of the level of soil hydration/encrustation, the amount of soil, and the mass of items being cleaned. Claims as to enzymatic enzyme detergent products “cleaning within minutes” are fallacious without specifics as to the above parameters.
Enzyme Surgical Instrument Cleaner Detergent
Types of enzymes potentially used within enzymatic enzyme detergents: Lipase Enzymes: (to breakdown fat) to cleave fatty acid residue from the glycerol residue in a neutral fat or a phospholipid. Amylase Enzymes: (to breakdown starch) to catalyze the hydrolysis of starch to sugar to produce carbohydrate derivatives. Carbohydrase Enzymes: (to breakdown starch to a lower level) to catalyze the hydrolysis of higher carbohydrates to lower forms. Protease Enzymes: (breakdown blood) including the proteinases and peptidases, to catalyze the hydrolytic breakdown of proteins. The use of enzymatic enzyme detergents allows lower temperatures and shorter periods of mechanical cleaning to be employed, often after a preliminary period of soaking. In general, enzyme detergents remove protein from clothes soiled with blood, milk, sweat, grass, etc. far more effectively than non-enzyme detergents. At present only proteases and amylases are commonly used. Although a wide range of lipases is known, it is only very recently that lipases suitable for use in detergent preparations have been deployed.
Enzyme Surgical Instrument Cleaner Detergent
enzymatic enzyme detergent
surgical instrument cleaners
Enzymatic enzyme detergent surgical instrument cleaners must be cost-effective and safe to use. Early attempts to use proteases floundered because users developed hypersensitivity. This was combated by developing dust-free granulates (about 0.5 mm in diameter) in which the enzyme is incorporated into an inner core, containing inorganic salts (e.g. NaCI) and sugars as preservative, bound with reinforcing, fibers of carboxymethyl cellulose or similar protective colloid. This core is coated with inert waxy materials made from paraffin oil or polyethylene glycol plus various hydrophilic binders, which later disperse in the wash. This combination of materials both prevents dust formation and protects the enzymes against damage by other detergent components during storage. It has been reported that spraying enzymatic enzyme detergent surgical instrument cleaning products has resulted in respiratory irritations. It is suspected that this is due to the enzyme aerosols. Applying the enzymatic enzyme detergent in a liquid of foam state should avoid this potential complication. 
John Temple  Product Development  
Endoscope Cleaners
Surgical Instrument Cleaning Brushes
Endoscope Cleaning Brushes 
Enzyme Detergent Surgical Instrument Detergent
enzymatic enzyme detergent
surgical instrument cleaners
Surgical Instrument Cleaner with Conditioners product is used worldwide for cleaning surgical instruments cleaner. This cleaner is 100% biodegradable and Neutral pH. This Surgical Instrument Cleaner complies with Surgical Instrument and Surgical Instrument Washer specifications.
The enzymes used for enzymatic enzyme detergents are produced using species of Bacillus, mainly by just two companies. Novo Industri A/S produce and supply three proteases, Alcalase, from B. licheniformis, Esperase, from an alkalophilic strain of a B. licheniformis and Savinase, from an alkalophilic strain of B. amyloliquefaciens (often mistakenly attributed to B. subtilis). GistBrocades produce and supply Maxatase, from B. licheniformis. Alcalase and Maxatase (both mainly subtilisin) are recommended for use at 10-65°C and pH 7-10.5. Savinase and Esperase may be used at up to pH 11 and 12, respectively. The a-amylase supplied for detergent use is Termamyl, the enzyme from B. licheniformis which is also used in the production of glucose syrups. a-Amylase is particularly useful in washing glassware and with de-starching detergents.
Enzyme Detergent Surgical Instrument Detergent
enzymatic enzyme detergent
surgical instrument cleaners
Enzymatic enzyme detergent surgical instrument cleaners, in a non-ionic surfactant, are available for formulating in liquid 'spotting' concentrates, and are typically used for removing stubborn stains. Preparations containing both Termamyl and Alcalase are produced. Termamyl is sufficiently resistant to proteolysis to retain activity long enough to fulfill its cleaning function. It should be noted that all the enzymatic enzyme detergent proteolytic enzyme surgical instrument cleaners described are non-specific serine endoproteases, giving preferred cleavage on the carboxyl side of hydrophobic amino acid residues but capable of hydrolysing most peptide links.
Surgical Instrument Cleaning Detergent Lubricants cut cleaning costs.
Enzyme Detergent Surgical Instrument Detergent
There are opportunities to extend the use of enzymatic enzyme detergent surgical instrument cleaners. The recent availability of a suitable lipase may increase the quantities of enzymes employed. There are, perhaps, opportunities for enzymes such as glucose oxidase, lipoxygenase and glycerol oxidase as means of generating hydrogen peroxide in situ. Added peroxidases may aid the bleaching efficacy of this peroxide.
John Temple  Product Development

Enzyme Detergent Cleaners four Enzyme Detergents
A combination of enzyme cleaners and surfactant detergents are necessary for optimal surgical instrument cleaning. Enzyme Detergent surgical instrument cleaners are often referred to as all-in-one surgical instrument detergent enzyme lubricating cleaners. Surgical instrument cleaning enzymes are proteins produced by all living organisms that act as catalysts to speed up chemical reactions that would otherwise occur at a much slower rate. Catalysts delivered with the enzyme cleaners help the cleaning process from a beginning to an end. The enzyme catalysts are not used up in the reaction so they are available to help multiple reactions. Surgical instrument cleaning enzymes fit their target substrates like a lock fits a key. The surgical instrument cleaning enzymes active site is open only to specific target substances with a matching chemical and three dimensional shape. If the substrate doesn't fit, the surgical instrument cleaning enzymes can't enter and no cleaning reaction occurs. This makes the action of surgical instrument cleaning enzymes highly specific for their substrates. For this reason specific enzymes are needed for cleaning bioburden from surgical instruments. The four enzymes necessary for effective cleaning are: protease surgical instrument enzyme cleaners to breakdown proteins including blood, amylase surgical instrument enzyme cleaners to breakdown starch and catalyze the hydrolysis of starch, lipase surgical instrument enzyme cleaner to breakdown fats and high level lipids, and carbohydrase surgical instrument enzyme cleaner to breakdown high level starches. Enzyme detergents that do not offer these four enzymes cannot remove all forms of proteinaceous bioburden and will not be as effective for cleaning surgical instruments. When bioburden has been emulsified, i.e. during suction, the bioburden becomes more challenging to remove, particularly lipids and proteins. Highly concentrated protease enzyme cleaners and lipase enzyme cleaners are necessary.

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